Large-scale analysis of lysine SUMOylation by SUMO remnant immunoaffinity profiling
نویسندگان
چکیده
منابع مشابه
Uncovering the SUMOylation and ubiquitylation crosstalk in human cells using sequential peptide immunopurification
Crosstalk between the SUMO and ubiquitin pathways has recently been reported. However, no approach currently exists to determine the interrelationship between these modifications. Here, we report an optimized immunoaffinity method that permits the study of both protein ubiquitylation and SUMOylation from a single sample. This method enables the unprecedented identification of 10,388 SUMO sites ...
متن کاملLarge-scale content profiling for preservation analysis
The starting point of any operational endeavor to preserve digital content is gaining a deep understanding of the characteristics of the objects. Systematic analysis of digital object sets and the identification of sample objects that are representative of a collection are critical steps towards preservation operations and a fundamental enabler for successful preservation planning: Without a fu...
متن کاملSUMOhunt: Combining Spatial Staging between Lysine and SUMO with Random Forests to Predict SUMOylation
Modification with SUMO protein has many key roles in eukaryotic systems which renders the identification of its target proteins and sites of considerable importance. Information regarding the SUMOylation of a protein may tell us about its subcellular localization, function, and spatial orientation. This modification occurs at particular and not all lysine residues in a given protein. In competi...
متن کاملMapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.
SUMOylation is an essential ubiquitin-like modification involved in important biological processes in eukaryotic cells. Identification of small ubiquitin-related modifier (SUMO)-conjugated residues in proteins is critical for understanding the role of SUMOylation but remains experimentally challenging. We have set up a powerful and high-throughput method combining quantitative proteomics and pe...
متن کاملLarge-scale cytological profiling for functional analysis of bioactive compounds.
Cytological profiling (CP) is an unbiased image-based screening technique that uses automated microscopy and image analysis to profile compounds based on numerous quantifiable phenotypic features. We used CP to evaluate a library of nearly 500 compounds with documented mechanisms of action (MOAs) spanning a wide range of biological pathways. We developed informatics techniques for generating do...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2014
ISSN: 2041-1723
DOI: 10.1038/ncomms6409